Analysis Note

ControlRat cerebellum tissue lysate and human cerebellum tissue lysate.

Application

Research CategoryNeuroscience

Research Sub CategoryNeurotransmitters & Receptors

Anti-Glutamate Receptor Delta 1/2 Antibody detects level of Glutamate Receptor Delta 1/2 & has been published & validated for use in WB.

Western Blotting:
Human Cerebellum Lysate.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The majority of rapid excitatory transmission in the nervous system is mediated by glutamate-activated ion channels. Ionotropic glutamate receptors have been subdivided pharmacologically into AMPA (cx-amino-3-hydroxy-5methyl-Cisoxazole propionic acid), KA (kainate), and NMDA subtypes (Monaghan et al., 1989). These receptors have been implicated in a number of physiological and pathological processes. During development glutamate receptors are involved in neuronal differentiation, migration, and activity-dependent synapse formation (Balazs et al., 1989; Brewer, 1989; Komoru and Rakic, 1993). In mature neurons, long-term potentiation and long-term depression require ionotropic glutamate receptors. Glutamate-gated ion channels also play a central role in mediating the excitotoxic effects of glutamate (Olney, 1990). Pathophysiological processes thought to involve glutamate excitotoxicity and neurodegeneration include epilepsy stroke, Parkinson’s disease, Alzheimer’s disease, Huntington’s disease, and amyotrophic lateral sclerosis.

Immunogen

Synthetic linear peptide to the C-Terminus, conjugated to BSA.

Epitope: C-Terminus, Cytoplasmic

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified in 0.1M Tris-Glycine (pH7.4) 150mM NaCl with 0.05% NaN3.

Antigen Affinity Purified

Quality

Western Blotting: 1:5,000 dilution from a previous lot detected Glutamate Receptor Delta 1/2 on 10 µg of rat or human cerebellum lysate.

Specificity

Cat. # AB2285 reacts with the C-Terminus of Glutamate Receptor Delta 1/2.

Reacts weakly with mouse. May react with Primate based on sequence homology. Reactivity with other species has not been tested.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~114 kDa